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A concise overview of CRISPR-Cas9 and what it enables. Updated with key dates and specifics.
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# CRISPR-Cas9: Genome Editing Basics CRISPR-Cas9 is a technology adapted from a bacterial immune system for editing DNA. - Components: Guide RNA (gRNA) targets a DNA sequence; Cas9 is a nuclease that cuts DNA. - Mechanism: Cas9 induces a double-strand break near a PAM site; cells repair via NHEJ or HDR. - Applications: Research, model organisms, diagnostics; clinical applications are under active study. - Milestones: First demonstrations in eukaryotic cells published in 2013. Note: This entry is part of a seeded demo to exercise version history.
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